Difference between revisions of "1. Introduction"
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| − | + | The goal of this hands-on session is to perform some basic tasks in the preliminary and downstream analysis of RNA-seq data. You will start from RNA-seq reads originating from two zebrafish embryos at two different development stages. | |
| + | You will learn how to check read quality, perform read trimming, align the trimmed reads to the zebrafish genome, perform transcriptome reconstruction and compare the gene expression between the two different conditions, how to characterize the list of differentially expressed genes, how to reconstruct the transcriptome using a de novo assembly approach, and how to identify genomic variants in your data. | ||
Revision as of 19:25, 24 January 2016
The goal of this hands-on session is to perform some basic tasks in the preliminary and downstream analysis of RNA-seq data. You will start from RNA-seq reads originating from two zebrafish embryos at two different development stages. You will learn how to check read quality, perform read trimming, align the trimmed reads to the zebrafish genome, perform transcriptome reconstruction and compare the gene expression between the two different conditions, how to characterize the list of differentially expressed genes, how to reconstruct the transcriptome using a de novo assembly approach, and how to identify genomic variants in your data.
